FastQCFastQC Report
Mon 6 Feb 2017
SRR2077200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492118
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.042.9474261
GTATCAA78100.036.972691
ATCAACG103750.027.0635663
TCAACGC103450.027.0089994
TATCAAC105250.026.9071032
CAACGCA106100.026.3016015
AACGCAG110050.025.2324966
ACGCAGA125450.021.7612727
CGCAGAG126650.021.4072258
AGAGTAC126500.020.28619211
GAGTACT99950.019.78961212
CAGAGTA134550.019.50795610
GCAGAGT140300.019.0040459
AGTACTT106100.018.70736713
GTACTTT109400.018.3354114
GTACATG73600.018.096861
TACATGG72700.017.560172
TACTTTT125800.016.17872815
GGACCGT4050.016.1426686
ACTTTTT129200.016.04728916
Produced by FastQC (version 0.11.5)