FastQCFastQC Report
Mon 6 Feb 2017
SRR2077199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3506970
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.033.685361
GTATCAA54600.031.6910441
TCAACGC62800.026.2186814
ATCAACG62700.026.1526343
CAACGCA63950.025.6924845
TATCAAC67150.025.0505852
AACGCAG68000.024.2106086
ACGCAGA77800.020.8504037
CGCAGAG80250.019.91458
GTACATG83050.019.58971
TACATGG83900.018.8990482
AGAGTAC79250.018.79597911
GTGTAGC17350.018.0787091
GAGTACT44300.017.98021712
CAGAGTA85800.017.51383410
ACATGGG86400.017.4605143
GCAGAGT90450.016.8673639
TAGCCCT20050.016.1832454
AGTACTT49900.015.75644413
GACCGTA1554.5133944E-415.5870017
Produced by FastQC (version 0.11.5)