FastQCFastQC Report
Mon 6 Feb 2017
SRR2077199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3506970
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.038.450361
GTATCAA75300.035.6494641
TATCAAC100100.026.3280032
ATCAACG99350.026.3189723
TCAACGC99350.026.215084
CAACGCA102250.025.3358965
AACGCAG106650.024.00036
ACGCAGA122600.020.4938017
CGCAGAG124850.020.0016528
GAGTACT89050.018.9625712
GTACATG81600.018.4731
AGAGTAC125300.018.36219811
TAACGCG950.00800106818.09298122
GTGGTAT18250.017.914891
AGTACTT95650.017.90581113
CAGAGTA132050.017.86760310
TACATGG81800.017.6652242
GTACTTT98100.017.53674714
GCAGAGT138600.017.49529
ACATGGG85500.016.4178873
Produced by FastQC (version 0.11.5)