FastQCFastQC Report
Mon 6 Feb 2017
SRR2077198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062863
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43100.032.2251741
GGTATCA21500.029.3345931
TCAACGC48600.027.8414
ATCAACG50050.027.1727943
CAACGCA50750.026.5926445
AACGCAG51950.025.7773696
TATCAAC52650.025.6611712
ACGCAGA61350.021.6590087
CGCAGAG63200.020.5345178
AGAGTAC63050.018.756911
CAGAGTA69000.017.68131310
GTACATG61300.017.6537361
GAGTACT38400.017.5087612
GCAGAGT72800.017.0215139
GTATAGC10150.016.6378611
TACATGG63350.016.4304352
GTGGTAT12100.016.2351741
ACATGGG63400.015.7888633
CCGTGCG2201.6142712E-615.6896189
GTACTTT43600.015.42282814
Produced by FastQC (version 0.11.5)