FastQCFastQC Report
Mon 6 Feb 2017
SRR2077198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062863
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.038.5660361
GTATCAA68350.035.6564521
ATCAACG85200.027.9555033
TCAACGC85700.027.6719154
TATCAAC88350.026.9198282
CAACGCA87550.026.7329225
AACGCAG91000.025.530316
ACGCAGA105600.021.8448147
CGCAGAG106950.021.1958038
AGAGTAC105500.020.11505111
GAGTACT84200.020.01577412
CAGAGTA112650.019.32842310
GCAGAGT116750.018.7377329
AGTACTT91200.018.47917413
GTACTTT91150.018.34407214
GTGGTAT15650.017.5962071
TGGTATC15800.016.9917562
GTACATG59950.016.938551
TACATGG58850.016.200712
GTATAGC10650.016.1608351
Produced by FastQC (version 0.11.5)