FastQCFastQC Report
Mon 6 Feb 2017
SRR2077197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529973
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.031.9845521
GGTATCA16600.029.0719131
ATCAACG34700.027.4618553
TCAACGC35750.026.5571274
CAACGCA37650.025.3086245
TATCAAC38450.025.1014122
AACGCAG38600.024.7737126
ACGCAGA46400.019.9395547
CGCAGAG46900.019.4337028
GAGTACT28350.017.63624412
TACATGG45400.017.6144312
AGAGTAC47400.017.38644211
GTACATG48650.017.2886311
TAGCCCT14550.016.1350524
GCAGAGT53800.016.1064079
CAGAGTA52400.015.98432410
GTATAGG6050.015.9535111
ACATGGG47050.015.8505533
GTATTAG6400.015.6196641
ATACCGT1554.5046868E-415.5906776
Produced by FastQC (version 0.11.5)