FastQCFastQC Report
Mon 6 Feb 2017
SRR2077197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529973
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.039.332221
GTATCAA50450.035.5598751
ATCAACG64200.027.5656873
TCAACGC63900.027.4256944
CAACGCA66550.026.385355
TATCAAC67100.026.3743232
AACGCAG69900.025.1208156
ACGCAGA78700.021.9917187
CGCAGAG79750.021.5423668
AGAGTAC79400.020.29031411
GAGTACT61400.019.3429512
CAGAGTA85150.019.28476110
GCAGAGT88300.018.948819
AGTACTT65700.017.76259213
GTACTTT66400.017.68322214
GGACCGT1855.558053E-616.7498726
GTATAGC7650.016.654211
GTACATG47600.016.0594141
TACATGG46700.015.4088462
GTGCGCC5150.015.373898511
Produced by FastQC (version 0.11.5)