FastQCFastQC Report
Mon 6 Feb 2017
SRR2077196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334307
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50750.033.3443371
GGTATCA26750.032.3391
TCAACGC61500.026.771074
ATCAACG61950.026.3537433
TATCAAC63550.025.9782332
CAACGCA65250.025.3911975
AACGCAG67850.024.313186
ACGCAGA79600.020.5077657
CGCAGAG80100.020.0368678
GTACATG83000.018.2705331
AGAGTAC78600.018.11973811
TACATGG83350.017.6154462
GAGTACT42450.017.2222212
CAGAGTA84350.017.20333910
ACATGGG86450.016.7689863
GCAGAGT89300.016.6218229
GTGTAGC17800.015.4898281
GTATAGC11750.014.959221
AGTACTT49650.014.72540113
GTACTAG6100.014.6899441
Produced by FastQC (version 0.11.5)