FastQCFastQC Report
Mon 6 Feb 2017
SRR2077196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334307
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.040.9841581
GTATCAA77300.035.229381
TCAACGC100350.026.6197684
ATCAACG101200.026.6000163
TATCAAC101550.026.406772
CAACGCA102800.025.6501465
AACGCAG106950.024.6226966
TGGTATC15300.021.7962072
GTGGTAT16650.021.683751
ACGCAGA124500.020.7502487
CGCAGAG126800.020.250558
GAGTACT89150.019.39232812
AGAGTAC124700.019.2661111
CAGAGTA129700.018.63050810
GTACATG78800.018.4575251
AGTACTT94600.018.45677813
GTACTTT94600.018.24727214
GCAGAGT135200.018.2042089
TACATGG79400.017.5794872
TATACCG1603.043823E-517.1892765
Produced by FastQC (version 0.11.5)