FastQCFastQC Report
Mon 6 Feb 2017
SRR2077195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908647
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.038.6859051
GTATCAA52900.035.9573361
TCAACGC65600.028.4643064
ATCAACG67050.027.9531443
CAACGCA69050.027.3410915
TATCAAC69400.027.1634082
AACGCAG73200.025.6962746
ACGCAGA81600.022.7123137
CGCAGAG83100.022.1781318
GAGTACT44450.020.78841212
AGAGTAC80950.020.48667711
CAGAGTA86650.019.60671610
GCAGAGT90450.019.538499
GTACATG85200.019.373141
TACATGG85700.018.739652
GTACTTT50100.018.6527214
AACGTCG1301.18101394E-418.57856422
GTGGTAT12650.018.5234761
AGTACTT51750.018.2572713
ACATGGG86900.018.0329063
Produced by FastQC (version 0.11.5)