FastQCFastQC Report
Mon 6 Feb 2017
SRR2077195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908647
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.1695289940649381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31380.10788521260916158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.042.5422971
GTATCAA85200.037.296721
TATCAAC113600.027.725582
ATCAACG114900.027.3216383
TCAACGC115150.027.1428814
CAACGCA118650.026.2258385
AACGCAG122100.025.3158516
GTGGTAT14700.025.0325261
ATGCGCG1052.3084813E-522.96612751
ACGCAGA137500.022.210927
CGCAGAG138200.021.8362528
TGGTATC17250.020.9297312
AGAGTAC137300.020.0991111
GAGTACT105750.019.88853612
CAGAGTA143950.019.60100210
GCAGAGT147900.019.3580199
AGTACTT111950.019.03200113
GTACATG82250.018.773651
TACATGG81450.018.405982
GTACTTT115900.018.12399714
Produced by FastQC (version 0.11.5)