FastQCFastQC Report
Mon 6 Feb 2017
SRR2077194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863750
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44250.035.047521
GGTATCA22150.033.4520531
TCAACGC55200.026.8234394
ATCAACG55550.026.6558343
TATCAAC57350.026.1432422
CAACGCA57400.025.855045
AACGCAG59500.024.8243266
ACGCAGA68550.021.1438887
CGCAGAG70350.020.3085678
GTACATG72800.019.077951
AGAGTAC68550.018.4579611
TACATGG74150.018.1747822
GAGTACT36900.018.1256212
CAGAGTA73550.017.89659510
GCAGAGT77150.017.3114439
ACATGGG74550.017.2695183
GTGTAGC13650.016.9160981
AGTACTT42500.016.46882413
GTACTTT40450.016.45193714
GTGGTAT13450.014.8615391
Produced by FastQC (version 0.11.5)