FastQCFastQC Report
Mon 6 Feb 2017
SRR2077194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863750
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.10859886512439981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.040.44061
GTATCAA71300.039.547661
TATCAAC98350.028.070032
ATCAACG99250.027.8848573
TCAACGC98500.027.8176044
CAACGCA99700.027.3437275
AACGCAG103750.026.209986
ACGCAGA118800.022.6403987
CGCAGAG122400.021.906668
AGAGTAC120350.020.67601211
TGGTATC13750.020.5283492
GAGTACT91950.020.2497712
CAGAGTA126700.019.88493510
GTGGTAT15100.019.8366681
GCAGAGT130400.019.7169789
AGTACTT96850.019.18972213
GTACTTT98100.018.9183914
GTACATG69200.017.363841
TACTTTT110400.016.82593215
TACATGG69450.016.5049742
Produced by FastQC (version 0.11.5)