FastQCFastQC Report
Mon 6 Feb 2017
SRR2077193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905489
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40700.034.127261
GGTATCA19600.032.8833661
TCAACGC48950.027.226814
ATCAACG49250.027.1315363
CAACGCA49900.026.9843045
TATCAAC50350.026.8353522
AACGCAG52600.025.2661866
ACGCAGA60850.021.3864147
CGCAGAG62000.020.7702548
AGAGTAC62600.018.83647711
CAGAGTA67400.017.89715410
GCAGAGT70500.017.3848699
GTACATG61000.017.2894951
GAGTACT39350.016.99886312
TACATGG60300.016.4625022
ACATGGG61350.015.8147163
AGTACTT43300.015.60876313
GTACTTT42500.015.49689814
GTGTAGC16300.015.4356881
GTACACG3001.1952579E-814.9352621
Produced by FastQC (version 0.11.5)