FastQCFastQC Report
Mon 6 Feb 2017
SRR2077193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905489
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30050.038.354231
GTATCAA65700.037.808091
ATCAACG86100.027.6869833
TCAACGC86300.027.4235214
TATCAAC89000.027.0167272
CAACGCA88800.026.4946865
AACGCAG92550.025.0495016
ACGCAGA107000.021.3264377
CGCAGAG108250.020.7070528
GTGGTAT14100.019.7641831
AGAGTAC106050.019.73923911
GAGTACT84000.019.43739712
CAGAGTA112150.018.97306810
GCAGAGT116750.018.4924269
AGTACTT89000.018.34540713
GTACTTT89800.018.07492414
GTACATG60750.017.9525411
TGGTATC13950.017.014552
GTGTAGC16050.016.9342121
TACATGG61200.016.9184532
Produced by FastQC (version 0.11.5)