FastQCFastQC Report
Mon 6 Feb 2017
SRR2077192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930541
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42150.14383009826513263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.036.253151
GTATCAA70650.035.0321461
TATCAAC87400.027.7661532
CGCGAAC500.007736270327.60041421
ATCAACG87400.027.2925993
TCAACGC87700.027.1167984
CAACGCA89700.026.5506695
AACGCAG91800.025.941086
ACGCAGA104600.022.2717197
CGCAGAG106700.021.7047428
GAGTACT52450.020.24713312
AGAGTAC106150.020.13863611
GTACATG116400.020.1377261
TACATGG114450.019.5471462
AGTACTT55100.019.39884813
CAGAGTA114050.019.25037610
GCAGAGT115900.019.0295649
GTACTTT57800.018.55264314
GTGGTAT16350.018.3423921
ACATGGG118800.018.3063743
Produced by FastQC (version 0.11.5)