FastQCFastQC Report
Mon 6 Feb 2017
SRR2077192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930541
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66810.2279783835134878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.13881395960677567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38150.13018074137164437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55700.044.292611
GTATCAA116450.035.3393861
TATCAAC149600.027.0434112
TCAACGC153000.026.419064
ATCAACG153700.026.4106453
CAACGCA155150.025.8529575
AACGCAG158850.025.1641566
GTGGTAT20950.024.9647311
TGGTATC21600.021.501352
ACGCAGA185650.021.4401867
CGCAGAG185550.021.3006528
AGAGTAC180900.020.2872211
GAGTACT136600.020.14354912
GTACATG110100.020.0638871
TACATGG108700.019.8753992
GCAGAGT192100.019.7324319
AGTACTT138650.019.67206813
CAGAGTA191100.019.54635810
GGACCGT2204.3473847E-918.7638556
GTACTTT148150.018.5317614
Produced by FastQC (version 0.11.5)