FastQCFastQC Report
Mon 6 Feb 2017
SRR2077191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249383
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.035.419531
GTATCAA43250.033.7010351
ATCAACG52100.026.5009273
TCAACGC52350.026.3064044
CAACGCA54100.025.774455
TATCAAC55400.025.503312
AACGCAG55650.024.9933836
ACGTCGT1154.6082554E-520.99863823
ACGCAGA67050.020.1265747
CGCAGAG67450.019.8034578
GTACATG68700.018.708591
TACATGG68650.018.3220772
AGAGTAC67250.017.9453711
GAGTACT40150.017.43387212
GTATAGC12950.017.2954791
CAGAGTA72950.017.05313910
ACATGGG71600.016.824853
GCAGAGT78250.016.1890189
TAGCCCT21100.016.030574
AGTACTT47400.015.13265913
Produced by FastQC (version 0.11.5)