FastQCFastQC Report
Mon 6 Feb 2017
SRR2077191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249383
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.038.1935771
GTATCAA64050.036.6796341
ATCAACG82500.028.054613
TCAACGC82550.027.8709744
TATCAAC83500.027.7598112
CAACGCA84000.027.3071445
AACGCAG86750.026.1640036
ACGCAGA99850.022.1591387
CGCAGAG102950.021.275318
AGAGTAC100850.020.62475211
GAGTACT78400.019.73357412
CAGAGTA108100.019.59195110
GCAGAGT112950.019.0255189
AGTACTT84550.018.8674713
GTGGTAT16600.018.6490971
GTACTTT84800.018.57803314
GTACATG62950.017.7040521
TACATGG63700.016.9524842
TATACCG1653.951962E-516.6738455
TGGTATC17350.016.4521142
Produced by FastQC (version 0.11.5)