FastQCFastQC Report
Mon 6 Feb 2017
SRR2077190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702465
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35050.034.7137151
ATCAACG41150.028.6056043
TCAACGC41400.028.3463294
CAACGCA42600.027.7114325
TATCAAC43350.027.1126292
GGTATCA16300.026.8553331
AACGCAG45750.025.6506276
ACGCAGA51800.022.4556927
CGCAGAG52500.022.0272458
AGAGTAC54250.019.32223111
TACATGG52600.018.8062632
GTACATG55450.018.3994371
CAGAGTA58400.018.3529410
GCAGAGT61700.017.9025659
GAGTACT33700.017.70107312
ACATGGG56150.017.5211123
GTACTTT36750.016.14027214
AGTACTT38450.015.51519813
CATGGGG40350.015.3118534
GTGTAGC13100.014.7343551
Produced by FastQC (version 0.11.5)