FastQCFastQC Report
Mon 6 Feb 2017
SRR2077190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702465
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.039.8163761
GTATCAA52300.035.8466871
ATCAACG65850.027.4226473
TATCAAC67550.026.9871122
TCAACGC66300.026.9252474
CAACGCA68650.025.7530365
AACGCAG71700.024.6575456
GTGCGAC953.143008E-421.71081211
ACGCAGA82300.021.3365787
CGCAGAG83500.020.9180988
AGAGTAC83200.020.07989111
CAGAGTA87600.019.1911710
TAGCGCG1107.427284E-419.11123829
GAGTACT66600.018.99424412
GCAGAGT91900.018.3316449
ACCGTCG950.00799198218.0963658
GTACATG50450.018.0691991
GTACTTT70950.017.93356714
AGTACTT71150.017.68294513
TACGCTA3600.017.56438431
Produced by FastQC (version 0.11.5)