FastQCFastQC Report
Mon 6 Feb 2017
SRR2077189_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077189_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638291
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34050.034.6189771
GGTATCA16300.029.6036781
TCAACGC40550.028.4420474
ATCAACG40400.028.3810213
CAACGCA41250.027.9599275
TATCAAC44050.026.3717332
AACGCAG43950.025.9269876
ACGCAGA51950.021.6685097
CGCAGAG53050.020.9596488
AGAGTAC52600.019.2718511
GTACATG54650.018.0376971
ACCGCGT1150.001103992918.0163548
CAGAGTA58050.017.81179810
TACATGG53250.017.7372232
GCAGAGT60300.017.3525629
GAGTACT31450.017.3216812
ACATGGG56600.015.8645673
TAGCCCT14300.015.6957694
AGTACTT35250.015.55366713
GTGTAGC13100.015.5233681
Produced by FastQC (version 0.11.5)