FastQCFastQC Report
Mon 6 Feb 2017
SRR2077189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638291
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50050.036.453071
GGTATCA23400.035.4538571
TCAACGC63900.027.8464364
ATCAACG64300.027.8337863
TATCAAC65300.027.4602472
CAACGCA65700.027.0311345
AACGCAG67100.026.0055076
ACGCAGA75800.022.8234967
CGCAGAG77500.022.114798
AGAGTAC78950.020.83363511
GAGTACT60750.019.65497412
CAGAGTA84450.019.6001510
GCAGAGT88000.019.0451769
AGTACTT64100.018.94985813
GTACTTT66500.018.53100414
GTACATG54100.018.3255711
TACATGG53750.017.7370412
GGACCGT2251.13981514E-716.8263556
ACATGGG55200.016.7723183
TACTTTT78650.015.8328515
Produced by FastQC (version 0.11.5)