FastQCFastQC Report
Mon 6 Feb 2017
SRR2077188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573468
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.037.3609731
GTATCAA53100.034.206211
TCAACGC63900.027.4952324
ATCAACG65400.027.0783843
TATCAAC66700.026.457282
CAACGCA66550.026.3481435
AACGCAG70250.025.1053496
ACGCAGA82900.020.6912867
GTACATG78200.020.0095791
CGCAGAG85200.019.9318548
TACATGG78900.018.7841932
AGAGTAC82850.018.39789811
ACATGGG80350.017.7867893
GAGTACT50450.017.7735812
CAGAGTA88300.017.60299110
GCAGAGT96050.016.5681699
CATGGGG52650.015.3411514
GTACTTT58050.015.329535514
AGTACTT58400.015.17796313
CCTATAC8450.015.1156943
Produced by FastQC (version 0.11.5)