FastQCFastQC Report
Mon 6 Feb 2017
SRR2077188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573468
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.042.0298161
GTATCAA77750.035.2062641
ATCAACG99850.026.7203313
TATCAAC99900.026.6725432
TCAACGC99550.026.490024
CAACGCA101150.025.9343095
AACGCAG105300.024.9122076
ACGCAGA123100.020.8214517
CGCAGAG124950.020.1962038
GTGGTAT17850.019.6503011
AGAGTAC121400.019.50938411
GAGTACT91500.019.15985112
TGGTATC16700.019.1467322
CAGAGTA127900.018.89470110
GTACATG76600.018.765271
TACATGG74800.018.3400082
GTATAGC11600.018.083361
AGTACTT97700.017.94372413
GCAGAGT137600.017.8382919
GTACTTT99200.017.64540714
Produced by FastQC (version 0.11.5)