FastQCFastQC Report
Mon 6 Feb 2017
SRR2077187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256160
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45400.031.3537371
GGTATCA24300.026.381641
ATCAACG54550.024.933213
TCAACGC55050.024.7660264
CGGTCGA851.4492971E-424.34003349
TATCAAC58150.023.9467642
CAACGCA57500.023.5910855
AACGCAG59650.022.796546
ACGCAGA69150.019.4647527
CGATCGT1801.9858635E-719.16505642
CGCAGAG70800.018.7682578
AGAGTAC68500.017.71498111
GTACATG68050.017.372451
TACATGG67200.016.9775072
GTACCGT3501.8189894E-1216.7634726
GAGTACT42100.016.54085412
CAGAGTA77250.016.23745310
GCAGAGT80600.015.972659
ACATGGG68400.015.9480563
TAGCCCT20800.015.5984374
Produced by FastQC (version 0.11.5)