FastQCFastQC Report
Mon 6 Feb 2017
SRR2077187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256160
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79600.034.7031171
GGTATCA39300.034.575671
ATCAACG104250.026.030763
TCAACGC105500.025.6897374
TATCAAC106650.025.6388682
CAACGCA107900.025.0541885
AACGCAG111200.024.2797436
ACGCAGA127300.021.0310637
CGCAGAG128950.020.5623258
AGAGTAC123450.020.38977411
GAGTACT101650.020.16189412
AGTACTT107200.019.34260613
CAGAGTA134900.018.8123710
GTACTTT109800.018.76652314
GCAGAGT139400.018.3292719
GTACATG64450.016.9734541
TACTTTT124350.016.61349315
TACATGG62800.016.2115552
ACTTTTT133300.015.86343916
GTGGTAT21950.015.8289791
Produced by FastQC (version 0.11.5)