FastQCFastQC Report
Mon 6 Feb 2017
SRR2077186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759504
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39300.033.583631
ATCAACG45550.028.4992333
TCAACGC45700.028.32864
GGTATCA19050.028.2196081
CAACGCA47400.027.093115
TATCAAC47950.026.8828472
AACGCAG49600.025.5415386
ACGCAGA57650.021.5558857
CGCAGAG58150.021.2533598
CGCCTAA2002.8885552E-818.97288522
AGAGTAC56800.018.87894611
GTACATG61450.017.7209341
CAGAGTA62650.017.54811110
TACATGG61950.016.9687882
GCAGAGT67900.016.5237229
ACATGGG61650.016.4084783
GAGTACT32850.016.37430212
ATAGCGT2351.8083847E-716.1579956
GTATACG2351.8438368E-716.130441
GTGGTAT12550.015.1022031
Produced by FastQC (version 0.11.5)