FastQCFastQC Report
Mon 6 Feb 2017
SRR2077186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759504
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.038.940321
GTATCAA65100.037.062961
ATCAACG83750.027.733953
TCAACGC83450.027.5450084
TATCAAC85650.027.399952
CAACGCA85750.026.8854735
AACGCAG89750.025.3805226
ACGCAGA102550.021.8635757
CGCAGAG103850.021.506928
GAGTACT81200.020.97502912
AGAGTAC105050.020.17613811
CAACGCG1206.5199994E-520.0728744
CAGAGTA108850.019.69412210
AGTACTT87500.019.6614413
GCAGAGT111950.019.3349469
GTACTTT88500.019.17547414
AACGCGG1108.387681E-418.7687595
GTACATG61900.018.683221
TACATGG62500.017.5081352
TGGTATC12950.017.271752
Produced by FastQC (version 0.11.5)