FastQCFastQC Report
Mon 6 Feb 2017
SRR2077185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340301
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46350.033.2376371
GGTATCA24400.033.19331
TCAACGC58600.025.6204594
ATCAACG59450.025.373523
CAACGCA59800.025.0480865
TATCAAC61700.024.5786742
AACGCAG62250.023.8923476
ACGCAGA71350.020.458637
GTACATG68200.020.0621971
TACATGG67750.019.9928262
CGCAGAG72600.019.9171058
AGAGTAC68300.018.9291711
GAGTACT37750.018.81317112
ACATGGG70150.018.0588263
CAGAGTA75350.017.5608710
GCAGAGT78700.017.2350739
AGTACTT44150.016.47785413
GTACTTT44000.016.4556714
ACGCGAC1906.8976733E-616.37364460
GTACTAG3601.8189894E-1216.2749161
Produced by FastQC (version 0.11.5)