FastQCFastQC Report
Mon 6 Feb 2017
SRR2077185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340301
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.18053233323405837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.10720843173591774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24210.10344823165908999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.042.899491
GTATCAA78550.038.2647171
TATCAAC103750.028.733592
ATCAACG105100.028.2990783
TCAACGC105100.028.13554
CAACGCA107150.027.533035
AACGCAG109750.026.724126
ACGCAGA125000.023.2538157
CGCAGAG125550.022.8895368
AGAGTAC121750.022.27217511
GTGGTAT13750.022.2597621
TGGTATC13000.022.2174822
GAGTACT92500.021.92122812
CAGAGTA129050.021.36033610
GCAGAGT133700.020.901569
AGTACTT98000.020.69095413
GTACTTT101250.019.90038514
GTATAGG6950.018.8032111
TACTTTT110550.018.33930415
GTACATG68950.018.2549251
Produced by FastQC (version 0.11.5)