FastQCFastQC Report
Mon 6 Feb 2017
SRR2077184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670620
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42400.034.788661
GGTATCA20150.034.3780171
ATCAACG50850.027.9041393
TCAACGC51050.027.32044
TATCAAC53100.027.0027162
CAACGCA52200.026.785155
AACGCAG55550.024.7329526
CGCAGAG64650.020.77228
ACGCAGA65250.020.7387457
AGAGTAC62450.019.43487511
GAGTACT36350.018.30696312
TAGCCCT13450.018.223744
CAGAGTA69450.017.76555310
GTACATG60150.017.532571
GCAGAGT73200.017.4027029
GTATACG1602.9796405E-517.2317661
GTGTAGC11250.017.155181
ATAACGC2057.7077857E-716.8408433
TAACGCA1653.8232924E-516.738154
TACATGG60900.016.5828442
Produced by FastQC (version 0.11.5)