FastQCFastQC Report
Mon 6 Feb 2017
SRR2077184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670620
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.042.2310451
GTATCAA66350.033.6504941
TATCAAC86650.025.4838242
ATCAACG86950.025.1981093
TCAACGC86750.025.1372574
CAACGCA89000.024.463125
AACGCAG92350.023.6129636
GTGGTAT14400.021.2625431
TGGTATC13700.020.586922
ACGCAGA107250.020.0586097
CGCAGAG108500.019.773068
GCGTATC1056.142023E-419.6584661
AGAGTAC102500.019.48672111
GAGTACT77650.019.08192812
CAGAGTA111600.018.11406510
AGTACTT82900.017.99789213
GCAGAGT116050.017.7756379
GTACTTT86200.017.15674414
GTACATG60300.016.8873731
TACATGG60000.016.4523812
Produced by FastQC (version 0.11.5)