FastQCFastQC Report
Mon 6 Feb 2017
SRR2077183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674972
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45650.032.7623561
GGTATCA23700.032.134391
TCAACGC54000.026.204714
ATCAACG54800.026.0132313
TATCAAC57050.025.3716892
CAACGCA56700.024.9558475
AACGCAG60700.023.3652956
ACGCAGA68700.020.2936677
GTACATG72900.019.7121811
TACATGG70300.019.6092242
CGCAGAG70050.019.5590578
AGAGTAC67400.018.57344611
ACATGGG74900.017.4194643
CAGAGTA75450.016.94497110
GAGTACT40000.016.5538412
GCAGAGT79700.016.412559
AACGTCG1907.0121914E-616.3458336
CCGCACG1300.002531233715.915421551
CGTGCGC5000.015.85199710
GTGTAGC20650.015.5199051
Produced by FastQC (version 0.11.5)