FastQCFastQC Report
Mon 6 Feb 2017
SRR2077183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674972
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.038.2480741
GTATCAA62650.034.2534331
TCAACGC80600.026.0646634
ATCAACG81450.025.834873
TATCAAC84300.025.1657242
CAACGCA83700.024.8114135
AACGCAG89350.023.0115916
ACGCAGA102550.019.8011427
CGCAGAG101450.019.7244578
AGAGTAC102600.018.32524711
GTATACG1954.755493E-717.636241
GTACATG72100.017.5053731
CAGAGTA109900.017.23499310
TACGACC1603.0404519E-517.1915864
GAGTACT77200.017.14167812
TACATGG70000.016.8971042
GCAGAGT114650.016.7917069
AACGCCG2058.046918E-716.772055
CGCTATC3551.8189894E-1216.56696533
AGTACTT83350.016.08306913
Produced by FastQC (version 0.11.5)