FastQCFastQC Report
Mon 6 Feb 2017
SRR2077182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963040
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.033.8308641
GTATCAA40300.033.6138651
ATCAACG49550.026.5511593
TCAACGC48900.026.5487674
CAACGCA51050.025.3630185
TATCAAC53600.024.3740312
AACGCAG53400.024.1142756
ACGCAGA60450.020.902177
CGCAGAG60900.020.4086328
GTACATG59050.019.321421
CGTGCGC3650.018.88690210
AGAGTAC63150.018.5130311
TACATGG58550.018.4276682
GAGTACT37200.017.70686112
CAGAGTA68200.017.18375810
ACATGGG62550.016.7269763
GCAGAGT72400.016.595829
TAGCCCT18500.015.67736154
GTACTTT41800.015.42902614
GTATAGC10850.014.9313491
Produced by FastQC (version 0.11.5)