FastQCFastQC Report
Mon 6 Feb 2017
SRR2077182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963040
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.040.954111
GTATCAA55900.034.5835651
TATCAAC73950.025.7640462
TCAACGC73750.025.5536924
ATCAACG74400.025.3766653
CAACGCA74750.024.9818045
AACGCAG79150.023.506156
ACGCAGA89700.020.4164927
CGCAGAG92050.019.6455888
AGAGTAC91500.018.74665611
GAGTACT68150.018.66294912
CAGAGTA94700.018.1504110
GTGGTAT14800.018.1291791
GCAGAGT99500.017.4824059
TGGTATC13500.017.322782
AGTACTT74450.017.22219513
GTACTTT75850.017.13554814
GTACATG59200.016.9670521
TACATGG59000.016.9039612
ACATGGG60950.016.0243263
Produced by FastQC (version 0.11.5)