FastQCFastQC Report
Mon 6 Feb 2017
SRR2077181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324083
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.032.3160481
GTATCAA31800.032.078691
TCAACGC37850.026.0822474
ATCAACG38400.025.983393
TATCAAC39000.025.5395552
CAACGCA38450.025.3166965
AACGCAG41700.023.0109676
GTACATG46500.020.2330681
ACGCAGA47750.019.7344387
CGCAGAG48000.019.5614328
TACATGG46750.019.5363922
ACATGGG47700.018.1670553
AGAGTAC46400.017.98349211
CAGAGTA50750.016.9757110
GCAGAGT53700.016.1351289
GTGTAGC11250.015.6231881
GAGTACT29600.015.26005912
CATGGGG32350.015.0449124
GTGTACG2302.6051948E-614.9838771
GTATAGG5750.014.9838771
Produced by FastQC (version 0.11.5)