FastQCFastQC Report
Mon 6 Feb 2017
SRR2077181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324083
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.036.019211
GTATCAA42800.034.9982221
ATCAACG55350.026.9976393
TATACCG907.0178903E-626.7799175
TATCAAC55350.026.6866072
TCAACGC56000.026.6842754
CAACGCA58000.025.7047635
AACGCAG59800.024.5855736
ACGCAGA69100.021.0573867
TACGCGT1154.720911E-520.93248764
CGCAGAG69400.020.779288
GAGTACT47550.020.26235612
AGAGTAC67700.019.72041911
CAGAGTA72350.018.73993710
GTATAGC7250.018.5236721
AGTACTT52550.018.46540313
CGTGCGC2053.9412043E-818.46499810
CGTCGTA950.00795297618.11160310
GTACATG51050.017.8077341
GCAGAGT76900.017.677799
Produced by FastQC (version 0.11.5)