FastQCFastQC Report
Mon 6 Feb 2017
SRR2077180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276680
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47250.030.2681581
TCAACGC54800.025.6336754
ATCAACG55100.025.3699953
CAACGCA57800.024.1841495
GGTATCA25650.024.0494631
AACGCAG59450.023.6261546
TATCAAC60400.023.4518852
TATACCG2553.6379788E-1220.3027425
ACGCAGA68300.019.8573977
CGCAGAG68800.019.3135748
AGAGTAC61750.018.87260211
GTACATG67800.018.6541771
TACATGG63300.018.5662232
GAGTACT37650.017.85781712
GTGTAGC16400.017.230971
ACATGGG65300.017.1785763
GTATAGC11250.017.1543881
GCAGAGT74950.016.5317889
CAGAGTA74650.016.34229910
GTACTTT40950.016.08512714
Produced by FastQC (version 0.11.5)