FastQCFastQC Report
Mon 6 Feb 2017
SRR2077180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276680
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75000.033.86881
GGTATCA38750.031.443891
ATCAACG94950.026.3860723
TATCAAC96150.026.1283452
TCAACGC95800.025.8286514
CAACGCA99850.024.711715
AACGCAG101650.024.1386996
ACGCAGA115050.020.9786477
CGCAGAG115050.020.75328
AGAGTAC108650.020.45104611
GAGTACT86000.020.15787912
ACCGTGC3600.020.0691538
AGTACTT91650.018.91519413
GTACTTT92500.018.86013614
CAGAGTA121700.018.82562810
GTACATG66950.018.6107031
GTATACG1301.20322584E-418.5335851
GCAGAGT125550.018.5229039
TACATGG64950.018.1741182
ACATGGG65900.017.3899043
Produced by FastQC (version 0.11.5)