FastQCFastQC Report
Mon 6 Feb 2017
SRR2077179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736513
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.033.8984951
GTATCAA39950.031.3985711
ATCAACG45600.026.1910083
TCAACGC45550.026.0667654
CAACGCA47350.025.077225
TATCAAC49200.024.6566832
ATACCGT1701.6734703E-1024.3640466
AACGCAG50050.023.9299876
ACGCAGA56600.020.7948117
CGCAGAG57200.020.3971568
AGAGTAC57300.018.65838811
AATACCG1552.238022E-517.8155525
TAGCCCT18150.017.3054074
GTGTAGC15800.017.2303981
CAGAGTA63900.017.15107210
GTACATG53850.017.086411
TATGCCG1250.001934914316.5684625
TATACTG12300.016.5572385
GCAGAGT69000.016.1094079
TACATGG53700.016.1085532
Produced by FastQC (version 0.11.5)