FastQCFastQC Report
Mon 6 Feb 2017
SRR2077179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736513
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.038.389561
GTATCAA59800.036.310671
ATCAACG76350.027.9388943
TCAACGC77200.027.3633774
TATCAAC79600.026.9278432
CAACGCA79350.026.5352465
AACGCAG81350.025.6291226
ACGCAGA92750.022.0133447
CGCAGAG94100.021.4555668
TATGCCG3000.020.80773543
GTGGTAT15400.020.781091
TGGTATC13600.020.4914512
AGAGTAC94000.020.12124611
CAGAGTA101700.019.03913310
GCAGAGT104100.018.7653779
TATCTCG3001.8189894E-1218.37207236
TACATGG51550.018.3539962
GAGTACT74600.018.30086712
GTACATG53700.018.263191
CTCGTAT3103.6379788E-1217.77909939
Produced by FastQC (version 0.11.5)