FastQCFastQC Report
Mon 6 Feb 2017
SRR2077178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21400
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAAGC150.002271426469.39764467
ACAGACT150.002335905868.911228
TGGTATC252.526528E-455.128972
AAGCTGG200.007230906351.80444756
CGAAGAG200.007298331751.6834078
CAGACTT200.007298331751.6834079
CACAGAC200.007298331751.6834077
GGTATCA851.8189894E-1244.5896071
GTGGTAT350.001326792539.3778341
GTATCAA2100.036.096351
AAGCAGA450.004551668730.6272076
TATCAAC2750.026.3115562
TCAACGC2900.026.1387374
ATCAACG3050.025.9829163
AACGCAG3300.025.0586246
CAACGCA3100.024.4523685
CGCAGAG3400.023.3082058
ACGCAGA3500.022.6422587
AGAGTAC3300.021.97764611
CAGAGTA3350.021.59903710
Produced by FastQC (version 0.11.5)