FastQCFastQC Report
Mon 6 Feb 2017
SRR2077178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21400
Sequences flagged as poor quality0
Sequence length75
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200.007471352351.3776361
TCTAAGA200.007471352351.3776362
CAGGGGT200.007541013451.2575959
TCTCCGA405.0490038E-542.8146971
GCTTGAA459.038804E-538.7841446
CGTATGC501.5429838E-435.4128331
TCGTATG501.5429838E-435.4128330
GTATGCC501.6568162E-434.9892332
GTACATG400.002631482734.251761
CTCCGAG501.8791985E-434.2517552
CCACGAG501.90536E-434.17172610
CACGAGA501.90536E-434.17172611
ACGAGAC501.90536E-434.17172612
TGCTTGA552.941195E-431.7324845
TGCCGTC553.0250152E-431.58173435
TATGCCG553.0676587E-431.50689333
GCCGTCT553.0676587E-431.50689336
CTTGAAA553.1107976E-431.43241147
CTCGTAT450.004170244631.17612629
CCCACGA553.3340766E-431.0652089
Produced by FastQC (version 0.11.5)