FastQCFastQC Report
Mon 6 Feb 2017
SRR2077177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4172526
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60950.033.132381
GGTATCA30000.030.440611
TCAACGC72050.027.4467374
ATCAACG73350.027.0099413
CAACGCA75900.025.9180985
TATCAAC78800.025.4112222
AACGCAG78950.024.8279136
ACGCAGA91300.021.015657
CGCAGAG93550.020.4740458
AGAGTAC90350.018.7370411
GTACATG91900.017.999251
CAGAGTA99650.017.70667610
TACATGG90550.017.5844652
GAGTACT52700.017.3379812
GCAGAGT105950.017.0684939
ATACCGT2451.5843398E-816.902826
ACATGGG95200.016.5688023
AGTACTT58650.015.69795313
GTACTTT59300.015.2353214
TACCGTA2756.564005E-815.0586947
Produced by FastQC (version 0.11.5)