FastQCFastQC Report
Mon 6 Feb 2017
SRR2077177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4172526
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40750.038.8808251
GTATCAA86700.036.509231
TCAACGC115900.026.5971264
ATCAACG117750.026.5586623
TATCAAC119000.026.3955152
CAACGCA119950.025.6122265
AACGCAG125700.024.2765926
ACGCAGA146100.020.5407897
CGCAGAG147500.020.059558
GTACATG92150.019.0584561
AGAGTAC147400.018.9280611
GTGGTAT21300.018.878541
GAGTACT108250.018.7271512
CAGAGTA155550.018.04742610
TACATGG92700.017.9801352
GCAGAGT162700.017.5299139
AGTACTT115500.017.43264213
GTACTTT119200.017.10212314
ACATGGG95650.016.9942443
TGGTATC23200.015.7017772
Produced by FastQC (version 0.11.5)