FastQCFastQC Report
Mon 6 Feb 2017
SRR2077176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026241
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39800.034.3810461
GGTATCA18900.033.3734361
TCAACGC45700.028.0272984
ATCAACG47050.027.2262743
CAACGCA48100.026.7006235
TATCAAC49400.026.6540032
AACGCAG50850.025.3894796
ACGCAGA59850.021.4561587
CGCAGAG60950.021.0703228
AACGTCG1004.3339957E-420.70216622
GTACATG62700.019.790041
AGAGTAC60450.019.6224611
CAGAGTA66450.018.25614510
TACATGG64900.018.2169422
TATACTG12600.017.5360985
GAGTACT35400.017.33836212
GCAGAGT72600.016.8811059
ACATGGG67850.016.7424873
GTGTAGC15550.016.1809561
TAGCCCT17650.015.8439644
Produced by FastQC (version 0.11.5)