FastQCFastQC Report
Mon 6 Feb 2017
SRR2077176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026241
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.037.003391
GTATCAA60600.036.6661151
ATCAACG76800.028.077673
TCAACGC76350.028.0629754
TATCAAC77450.027.975242
CAACGCA78650.027.1102355
AACGCAG81650.026.0299076
ACGCAGA96150.021.9446747
CGCAGAG99050.021.2450758
GTACATG67700.020.4748821
AGAGTAC97000.020.34537111
GAGTACT69850.020.13178612
TACATGG68450.019.9467322
CAGAGTA102200.019.37778910
GCAGAGT107550.018.8614129
AGTACTT76250.018.7125813
GTGGTAT14300.018.2802681
GTACTTT76750.018.19261714
AACGCGA950.008030917518.081265
ACATGGG70650.017.913913
Produced by FastQC (version 0.11.5)