FastQCFastQC Report
Mon 6 Feb 2017
SRR2077175_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077175_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534993
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.035.171591
GTATCAA39400.033.1490821
TCAACGC47050.027.5836624
ATCAACG47200.027.5008953
CAACGCA49250.026.4215815
TATCAAC48800.026.4122872
AACGCAG52750.024.5366526
ACGCAGA60050.021.2094047
CGCAGAG60050.021.0952828
AGAGTAC59350.019.7503811
GTATAGG5000.019.2981871
GTACATG59700.019.1065461
CGATCGT1108.2417444E-418.81774542
GAGTACT32900.018.33831212
TACATGG59450.018.3184812
CAGAGTA65900.017.98735210
GCAGAGT68850.017.3966799
ACATGGG61650.017.079213
AGTACTT36100.016.52436413
CATGGGG38200.015.9931354
Produced by FastQC (version 0.11.5)